Coding

Part:BBa_K4649001:Design

Designed by: Maxwell Swann   Group: iGEM23_EastCoastBioCrew   (2023-09-09)


gcd codon optimized for Rhizobium tropici


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1203
    Illegal BamHI site found at 1152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1203
    Illegal NgoMIV site found at 136
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 465
    Illegal NgoMIV site found at 504
    Illegal NgoMIV site found at 1998
    Illegal NgoMIV site found at 2155
    Illegal NgoMIV site found at 2240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Rhizobium tropici


Source

Escherichia coli strain K12 genome (UniProt accession: P15877)

References